First Illumina run for WP6 completed with success

Massive sequencing of microbial sequences from sponges for WP6  has started. Jon Bent Kristofersen who constructed the MiSeq Illumina library of the 16S barcode, unravelling the bacterial microsymbionts, is a specialist of next-generation sequencing technologies. He used a new “trick” to increase the quality of sequences, by adding the so-called ‘diversity spacers’ in the standard 16S primers. Quality control confirmed his expectations!